Guida degli insegnamenti

Syllabus

Partially translatedTradotto parzialmente
[W000606] - ENZYMOLOGY IN FOOD PROCESSINGENZYMOLOGY IN FOOD PROCESSING
Michele CIANCI
Lingua di erogazione: INGLESELessons taught in: ENGLISH
Laurea Magistrale - [AM04] FOOD AND BEVERAGE INNOVATION AND MANAGEMENT Master Degree (2 years) - [AM04] FOOD AND BEVERAGE INNOVATION AND MANAGEMENT
Dipartimento: [040027] Dip.Scienze Agrarie,Alimentari e AmbientaliDepartment: [040027] Dip.Scienze Agrarie,Alimentari e Ambientali
Anno di corsoDegree programme year : 2 - Primo Semestre
Anno offertaAcademic year: 2019-2020
Anno regolamentoAnno regolamento: 2018-2019
Obbligatorio
Crediti: 6
Ore di lezioneTeaching hours: 54
TipologiaType: B - Caratterizzante
Settore disciplinareAcademic discipline: BIO/10 - BIOCHIMICA

LINGUA INSEGNAMENTO LANGUAGE

INGLESE

English


PREREQUISITI PREREQUISITES

Good knowledge of Biochemistry, microbiology and food processing technology.

Good knowledge of Biochemistry, microbiology and food processing technology.


MODALITÀ DI SVOLGIMENTO DEL CORSO DEVELOPMENT OF THE COURSE

We foresee 4 ECTS (36 hours) of theoretical lessons, and 2 ECTS (18 hours) of critical reading and discussion of production protocols and scientific papers.

We foresee 4 ECTS (36 hours) of theoretical lessons, and 2 ECTS (18 hours) of critical reading and discussion of production protocols and scientific papers.


RISULTATI DI APPRENDIMENTO ATTESI LEARNING OUTCOMES
Knowledge and Understanding.

Mastering of the principles of enzymatic kinetics, main means for studying enzymology and biotechnological applications.


Capacity to apply Knowledge and Understanding.

Command of enzymatic stability and activity, to control enzymes and their applications to increase yield and quality in production or enhance product properties.


Transversal Skills.

Skills in designing and advising the appropriate deployment procedures during the different steps of food and beverage to enhance quality and yield; communication skills to transfer information, ideas and technical solutions to colleagues and customers.


Knowledge and Understanding.

Mastering of the principles of enzymatic kinetics, main means for studying enzymology and biotechnological applications.


Capacity to apply Knowledge and Understanding.

Command of enzymatic stability and activity, to control enzymes and their applications to increase yield and quality in production or enhance product properties.


Transversal Skills.

Skills in designing and advising the appropriate deployment procedures during the different steps of food and beverage to enhance quality and yield; communication skills to transfer information, ideas and technical solutions to colleagues and customers.



PROGRAMMA PROGRAM

Basics of Enzymology (1 ECTS): Enzyme Nomenclature and Classification. Isoenzymes. Enzyme structure. Active site. Enzyme biosynthesis, genetic information; structure-function relationship; solubility, denaturation, inactivation, stabilization; chemical and enzymatic modifications; enzyme engineering. Objectives and strategy of enzyme purification.

Enzymatic kinetics (0.75 ECTS): Michaelis-Menten’s kinetic law; kinetic parameters; specificity. Enzymatic inhibition; inhibitors, drugs, pesticides, herbicides. Arrhenius’ graph. Optimal working pH and temperature. Enzymatic catalysis and mechanism. Enzyme-substrate complex. Allosteric enzymes.

Production and industrial deployment of enzymes (0.75 ECTS): Sources and purification and modified enzymes. Enzymes and transgenic organism. Heterologous Enzymes; production of metabolites of agricultural, nutrimental and organoleptic interest. Immobilization, enzymatic reactors, multiple-enzyme processes, biosensors. Deployment of purified enzymes in agricultural and industrial processes. Qualitative, quantitative and economical aspects. European legislation about enzyme deployment in food production.

Quality control (0.5 ECTS). Quantification of enzymatic activity; enzymatic activity unit; specific activity; regulators of enzymatic activity. Quantification with support enzyme. Quantification of analytes of agricultural and food-farming interest. Enzymatic technology and biotechnology. Enzymes as quality markers.

Production (1.5 ECTS): Proteases, amylases, invertases, lipases; specificity, sources, applications: beer technology, enology, enhancement of nutrimental and functional qualities. Enzymes for lipidic matrices. Biotechnology of flavour production. Proteinaceous material recovery from production scraps. Current state and perspective in the use of enzymes of agricultural and food-farming interest

Enzymatic modification (1.5 ECTS): enzymes and their applications to increase yield and quality in production or enhance product properties; control and change of nutrimental and organoleptic characteristics. Enzymes action on carbohydrates: specificity, sources, application (sweetening, clearing, removal of unwanted flavours, stabilization). Polyphenol oxidases and lipoxygenases. Asparaginases and Transglutaminases.

Basics of Enzymology (1 ECTS): Enzyme Nomenclature and Classification. Isoenzymes. Enzyme structure. Active site. Enzyme biosynthesis, genetic information; structure-function relationship; solubility, denaturation, inactivation, stabilization; chemical and enzymatic modifications; enzyme engineering. Objectives and strategy of enzyme purification.

Enzymatic kinetics (0.75 ECTS): Michaelis-Menten’s kinetic law; kinetic parameters; specificity. Enzymatic inhibition; inhibitors, drugs, pesticides, herbicides. Arrhenius’ graph. Optimal working pH and temperature. Enzymatic catalysis and mechanism. Enzyme-substrate complex. Allosteric enzymes.

Production and industrial deployment of enzymes (0.75 ECTS): Sources and purification and modified enzymes. Enzymes and transgenic organism. Heterologous Enzymes; production of metabolites of agricultural, nutrimental and organoleptic interest. Immobilization, enzymatic reactors, multiple-enzyme processes, biosensors. Deployment of purified enzymes in agricultural and industrial processes. Qualitative, quantitative and economical aspects. European legislation about enzyme deployment in food production.

Quality control (0.5 ECTS). Quantification of enzymatic activity; enzymatic activity unit; specific activity; regulators of enzymatic activity. Quantification with support enzyme. Quantification of analytes of agricultural and food-farming interest. Enzymatic technology and biotechnology. Enzymes as quality markers.

Production (1.5 ECTS): Proteases, amylases, invertases, lipases; specificity, sources, applications: beer technology, enology, enhancement of nutrimental and functional qualities. Enzymes for lipidic matrices. Biotechnology of flavour production. Proteinaceous material recovery from production scraps. Current state and perspective in the use of enzymes of agricultural and food-farming interest

Enzymatic modification (1.5 ECTS): enzymes and their applications to increase yield and quality in production or enhance product properties; control and change of nutrimental and organoleptic characteristics. Enzymes action on carbohydrates: specificity, sources, application (sweetening, clearing, removal of unwanted flavours, stabilization). Polyphenol oxidases and lipoxygenases. Asparaginases and Transglutaminases.


MODALITÀ DI SVOLGIMENTO DELL'ESAME DEVELOPMENT OF THE EXAMINATION
Learning Evaluation Methods.

Based on the proficiency of the student during the written and/or oral discussion of three questions from the course program.


Learning Evaluation Criteria.

The student will have to demonstrate sound knowledge on (a) structure and function of enzymes, (b) the application to food processing and food quality control and (c) their production from gene to formulation.


Learning Measurement Criteria.

The final mark is given in thirtieths. Successful completion of the examination will lead to grades ranging from a minimum of 18 to a maximum of 30 cum laude.


Final Mark Allocation Criteria.

The verbal proof will have a positive score when the student show an overall knowledge of the topics, with a competent exposure. Deep knowledge of the topics will lead to the maximum marks. For each question 10 points are assigned.


Learning Evaluation Methods.

Based on the proficiency of the student during the written and/or oral discussion of three questions from the course program.


Learning Evaluation Criteria.

The student will have to demonstrate sound knowledge on (a) structure and function of enzymes, (b) the application to food processing and food quality control and (c) their production from gene to formulation.


Learning Measurement Criteria.

The final mark is given in thirtieths. Successful completion of the examination will lead to grades ranging from a minimum of 18 to a maximum of 30 cum laude.


Final Mark Allocation Criteria.

The verbal proof will have a positive score when the student show an overall knowledge of the topics, with a competent exposure. Deep knowledge of the topics will lead to the maximum marks. For each question 10 points are assigned.



TESTI CONSIGLIATI RECOMMENDED READING

P. Grunwald: Biocatalysis - Biochemical Fundamentals and Applications (ICP London, 2008).
R.J. Whitehurst, B.A. Law, eds.: Enzymes in Food Technology (Sheffield Academic Press, CRC Press, Sehffield, 2002). ISBN 1-84127-223-X
G.R. Whitaker, A.G.J. Voragen, DWS Wong Handbook of food enzymology. Marcel Dekker, Inc. New York-Basel (2002).

P. Grunwald: Biocatalysis - Biochemical Fundamentals and Applications (ICP London, 2008).
R.J. Whitehurst, B.A. Law, eds.: Enzymes in Food Technology (Sheffield Academic Press, CRC Press, Sehffield, 2002). ISBN 1-84127-223-X
G.R. Whitaker, A.G.J. Voragen, DWS Wong Handbook of food enzymology. Marcel Dekker, Inc. New York-Basel (2002).


E-LEARNING E-LEARNING

YES.
https://lms.univpm.it/course/view.php?id=1544

YES.
https://lms.univpm.it/course/view.php?id=1544


Scheda insegnamento erogato nell’A.A. 2019-2020
Le informazioni contenute nella presente scheda assumono carattere definitivo solo a partire dall'A.A. di effettiva erogazione dell'insegnamento.
Academic year 2019-2020

 


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